Question

Why does a plasmid need an origin of replication?

Answer 1

For example, the E. coli plasmidpBR322 uses a protein called Rop/Rom to regulate the number of plasmids that are within each bacterial cell. The most commonorigin of replication that is used inplasmids for genetic engineering is called pUC. This origin is derived from pBR322 but it contains two mutations.

Answer 2

The most common origin of replication that is used in plasmids for genetic engineering is called pUC. Thisorigin is derived from pBR322 but it contains two mutations.