Why does DNA move towards the anode in gel electrophoresis?
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Ans : To separate DNA using agarosegel electrophoresis, the DNA is loaded into pre-cast wells in thegel and a current applied. The phosphate backbone of the DNA(and RNA) molecule is negatively charged, therefore when placed in an electric field, DNAfragments will migrate to the positively charged anode.
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The negative charge on the sugar-phosphate backbone of DNA polymers cause them tomigrate towards the positive electrode when placed in an electrical field. ... For gel electrophoresis, DNA is placed in a porous gel.
To separate DNA using agarose gel electrophoresis, the DNA is loaded into pre-cast wells in the gel and a current applied. The phosphate backbone of the DNA (and RNA) molecule is negatively charged, therefore when placed in an electric field, DNA fragments will migrate to the positively charged anode.
To separate DNA using agarose gel electrophoresis, the DNA is loaded into pre-cast wells in the gel and a current applied. The phosphate backbone of the DNA (and RNA) molecule is negatively charged, therefore when placed in an electric field, DNA fragments will migrate to the positively charged anode.
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