Biology, asked by AyushSharma7775, 1 year ago

Why guanidium thiocyante l is used for rna separation ?

Answers

Answered by PawanBk
1

This method may take longer than a column-based system such as the silica-based purification, but has higher purity[citation needed] and the advantage of high recovery of RNA an RNA column is typically unsuitable for purification of short (<200 nucleotides) RNA species, such as siRNA, miRNA, gRNA and tRNA.

Answered by sg2544
0
heya answer is here........

♧The method may take longer than a column-based system such as the silica-based purification, but has higher purity[citation needed] and the advantage of high recovery of RNA: an RNA column is typically unsuitable for purification of short (<200 nucleotides) RNA species, such as siRNA, miRNA, gRNA and tRNA.

It was originally devised by Piotr Chomczynski and Nicoletta Sacchi and published in 1987.
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