Why ice cold ethanol needed in separating the DNA from the mixture?
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Ethanol needs to be incubated in the freezer for good results, this costs some time. But its easier to dry the sample afterwards, since ethanol is more volatile. ... Using chilled solvents is helpful since the solubility of DNA in the cold mixture is ways lower (hence the freezing step with EtOH) than at room-temperature.
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Using ice-cold ethanol and ice-coldwater increases the yield of DNA. Low temperatures protect the DNA by slowing down the activity of enzymes that could break it apart. ... DNases in the cytoplasm would destroy the DNAof viruses entering the cell. Cold ethanol helps the DNA to precipitate more quickl
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