Why introns are called intervening sequence? how do they eliminate?
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In eukaryotes, the gene contains extra sequences that do not code for protein. In these organisms, transcription of DNA produces pre-mRNA. These pre-mRNA transcripts often contain regions, called introns, that are intervening sequences which must be removed prior to translation by the process of splicing.
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Eukaryotic genes are often interrupted by sequences that do not appear in the final RNA. The intervening sequences that are removed are called "introns". ... The location of splice sites in a pre-mRNA can be determined by comparing the sequence of genomic DNA with that of the cDNA prepared from the corresponding mRNA.
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