Question

Why iron is used as chelating formin culture medium?

Answer 1

In this study, the effects of compounds providing Fe in chelated (NaFeEDTA and Fe(III)AC) and non-

chelated (FeSO4·7H2O) forms as components of culture media, on in vitro shoot multiplication and rooting of

Magnolia soulangeana ‘Alexandrina’, Magnolia grandiflora and Prunus cerasifera ‘Nigra’ were compara-

tively evaluated. Each of the tested chemicals was used as a single Fe source in the basal salt medium. In the

stages of shoot multiplication and rooting plant response was scored by biometrical indices (number of shoots,

leaves and roots, shoot and root length, percent of rooted plants and root hairs). The occurrence of physiolog-

ical disorders was estimated by visual observations. In presence of FeSO4, symptoms of chlorosis, hyperhy-

dricity, early senescence and specific morphology of roots, suggesting Fe deficiency, were observed. These

deteriorations were entirely prevented at the application of Fe chelates of which, in this experimental systems,

Fe(III)AC was tested for the first time. The addition of Fe(III)AC positively affected the plant quality to extent

comparable to that of NaFeEDTA. The obtained data suggest that both applied Fe chelates are more appro-

priate than non-chelated Fe form and can be alternatively used in the optimization of nutrient media for mi-

cropropagation of Magnolia and Prunus cerasifera genotypes.