Question

why is annealing in PCR also called hybridization​

Answer 1

Annealing of primers. To copy DNA, polymerases require a short sequence called a primer. ... They cannot "anneal" to the strand of DNA at temperature 95 degrees centigrade, so the test tube is cooled to 45 - 60 degrees C. The temperature of this step depends on the melting temperature of the primer - template hybrid.

Answer 2

Answer:

Annealing of primers. To copy DNA, polymerases require a short sequence called a primer. ... They cannot "anneal" to the strand of DNA at temperature 95 degrees centigrade, so the test tube is cooled to 45 - 60 degrees C. The temperature of this step depends on the melting temperature of the primer - template hybrid.

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