Why is continuous replenishment of oxaloaceti
acid required in TCA cycle?
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Answer:Use an oxaloacetate standard curve. Mix 0.2 ml. of each of the dilute oxaloacetic acid standard solutions with 1.0 ml. control buffer solution (III), equilibrate to 37 °C and add 1.0 ml. chromogen solution (II). After exactly 10 min. dilute with 5.0 ml. pyrosulphite solution (IV) and read the extinctions against the solution without oxaloacetic acid as described above.
Under the assay conditions the various oxaloacetic acid concentrations correspond to the following U/l.
Explanation:
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