why is limewater used in experiment to prove co2 is produced during respiratoin in germinating seeds
Answers
Answer:
when we pass co2 with lime water it becomes milky.... this is very easy and safe formula to check.....
and there should not be any reaction in process of germinate so co2 is used
- Germinate close to 25 seeds. This can be done by wrapping them in moist blotting paper or cotton wool for around 3 to 4 days
- Set up the germinated or sprouted seeds in the conical flask. Spray some water into the flask to dampen the seeds
- With the help of a thread, suspend the conical flask containing the test tube having a freshly prepared 20% KOH solution.
- Use the rubber cork to seal the opening of the conical flask.
- One edge of the U-shaped glass delivery tube present in the conical flask should be inserted through the hole in the rubber cork. The other edge should be placed into a beaker that is saturated with water
- All attachments of the set-up should be sealed. This can be done using vaseline to create an air-tight environment
- The initial water level present in the U-shaped delivery tube needs to be marked.
- Leave the experimental set-up uninterrupted for 1 to 2 hours. Observe the fluctuations in the water level in the tube.
Observation
Careful observation after a certain period of time reveals that the water level in the U-shaped delivery tube has risen in the beaker.
Conclusions
The rise in level water indicates that carbon dioxide is released as a result of germinating gram seeds during the process of respiration in the conical flask. The carbon dioxide that is released in the process is absorbed or consumed by the KOH solution that is suspended in the test tube in the conical flask, creating a vacuum or a void in the flask resulting in the upward water movement in the tube. Hence, the water level in the tube changes.