Question

Why isopropyl alcohol is used to determine fatty acid?

Answer 1

Liquid-liquid extraction of fatty acids from a vegetable oil is accomplished by aqueous isopropyl alcohol in the ratio of 1:1 in two-stage extractions at 30±2°C. The mixtures studied contained 0 to 50 fatty acids in groundnut oil, and aqueous isopropyl alcohol solutions were of 75, 80, 85, 95 and 99% concentrations. Phase separation depends on (i) the fatty acids content in the model mixture, and (ii) the concentration of aqueous isopropyl alcohol. Selective fatty acid separation and phase separation could be achieved by use of aqueous (75 and 80%) isopropyl alcohol over the whole range of fatty acid content in the model mixture.

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