Question

Why mRNA is being used instead of DNA in insulin reproduction?

Answer 1

Answer:

insert the human insulin gene into the plasmid. Researchers return the plasmid to the bacteria and… put the “recombinant” bacteria in large fermentation tanks. There, the recombinant bacteria use the gene to begin producing human insulin.

Answer 2

DNA has introns because splicing has not occurred, but mRNA just has the coding DNA, meaning that the entire molecule will code for insulin.

mRNA in insulin production:

• Preproinsulin, a single chain precursor, is produced during the translation of the insulin mRNA.
• Proinsulin is then produced as a result of the removal of its signal peptide upon insertion into the endoplasmic reticulum.
• An amino-terminal B chain is one of the three domains that make up proinsulin.
• Cap-independent INS mRNA translation contributes 40–100% of insulin production under stress conditions despite making up a tiny proportion of overall translation.
• A human gene could be inserted into the genetic code of a common bacterium thanks to a technique called recombinant DNA.
• The protein encoded by the human gene might now be produced by this "recombinant" microorganism.
• The human insulin gene is created in a laboratory.

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