Chemistry, asked by Moazzam676, 9 months ago

Why phenol chloroform is preferred than other solvent in dna extraction?

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Answered by Anonymous

1

Answer:

Why phenol chloroform is preferred than other solvent in dna extraction?

DNA is a polar molecule due to the negative charges on it's phosphate backbone, so it is very soluble in water and less so in phenol. This means that when the water(+DNA+protein) and phenol are mixed in the protocol, the DNA does not dissolve in thephenol, but remains in the water phase.

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