Why puc18 shows one band and pbr322 shows two bands in plasmid isolation by agarose gel electrophoresis?
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bands in a plasmid digest?
My lab students digested a recombinant pUC18 plasmid with EcoR1 restriction endonuclease. The plasmid had a 4878-bp segment of bacteriophage lambda inserted into the EcoR1 site, thus after digest and gel electrophoresis, two bands were expected -- one band for the plasmid (2686-bp) and one band for the insert (4878-bp). Instead, 4 bands appeared on the gel (see lanes 2, 5, 6, and 8 on the attached gel). Just the pUC18 plasmid (2686-bp) is in lanes 3 and 7. Any ideas why I am consistently seeing 4 bands
My lab students digested a recombinant pUC18 plasmid with EcoR1 restriction endonuclease. The plasmid had a 4878-bp segment of bacteriophage lambda inserted into the EcoR1 site, thus after digest and gel electrophoresis, two bands were expected -- one band for the plasmid (2686-bp) and one band for the insert (4878-bp). Instead, 4 bands appeared on the gel (see lanes 2, 5, 6, and 8 on the attached gel). Just the pUC18 plasmid (2686-bp) is in lanes 3 and 7. Any ideas why I am consistently seeing 4 bands
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