why scientist prefer cDNA library over gDNA library
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This way a protein expressed in a eukaryotic organism can be introduced into a prokaryote. For this process cDNA is used over gDNA, since prokaryotes cannot spice out introns contained in gDNA. In order to isolate cDNA, first the RNA of an organism must be isolated
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Scientists prefer cDNA library over gDNA library
Explanation:
- cDNA lack introns and therefore can be expressed in prokaryotic cells.
- Prokaryotes cannot spice introns present in gDNA.
- In order to isolate cDNA , RNA of an organism must be isolated.
- cDNA libraries are most useful in reverse genetics where additional genomic information is of no use.
- They are used in functional cloning for identification of genes based on the give data proteins function.
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