Why should spotting of mixture be done above the level of mobile phase?
Answers
Answered by
60
Answer:
because mobile has many harmful particles so we should not spot while doing experiments with mixtures
Answered by
71
Answer:
In thin-layer chromatography( TLC), the sample mixture should be spotted above the solvent level. It is because if the mixture gets in contact with the solvent initially, it dissolves and gets washed off in the solvent before traveling up the plate.
Ideally, the solvent layer should rise gradually by capillary action and along with it, the sample mixture should also travel up the thin layer chromatography plate.
Similar questions