Question

Why the red blood cells are not stained in the reticulocyte count stain?

Answer 1

Like mature red blood cells, in mammals reticulocytes do not have a cell nucleus. They are called reticulocytes because of a reticular (mesh-like) network of ribosomal RNA that becomes visible under a microscope with certain stains such as new methylene blue and Romanowsky stain.
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Answer 2

Reticulocytes are immature red blood cells, typically composing about 1% of the red blood cells in the human body. In the process oferythropoiesis (red blood cell formation), reticulocytes develop and mature in the bone marrow and then circulate for about a day in the blood stream before developing into mature red blood cells. Like mature red blood cells, in mammals reticulocytes do not have acell nucleus.