Question

Why there is a increase in uv absorption of single stranded dna?

Answer 1

the phenomenon of UV absorbance increasing as dna is denatured is known as hyperchromic shift. The puring and pyrimidine bases in dna strongly absorb ultraviolet light. Double- stranded DNA absorbs less strongly than denatured Dna due to stacking intersections between the bases

Answer 2

Answer:

The phenomenon of UV absorbance increasing as DNA is denatured is known as the hyperchromic shift. The purine and pyrimidine bases in DNA strongly absorb ultraviolet light. Double-stranded DNA absorbs less strongly than denatured DNA due to the stacking interactions between the bases.