Why we dont use electroporation for mammalian cells?
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Electroporation has now been shown to be effective at delivering plasmid DNA in vivo to a variety of tissue types. Electroporation makes use of the fact that the cell membrane acts as an electrical capacitor that is unable to pass current (except through ion channels).
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Electroporation—the use of high-voltage electric shocks to introduce DNA into cells—is a procedure that is gaining in popularity for standard gene transfer and also allows the generation of genetically modified mice
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