Question

Will a downstream heterozygous mutation affect a upstream termination mutation

Answer 1

Most proteins can withstand one or two point mutations before their function changes. Non-conservative mutations result in an amino acid change that has different properties than the wild type. The protein may lose its function, which can result in a disease in the organism.
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Answer 2

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Spontaneous mutations are the result of errors in natural biological processes, while induced mutations are due to agents in the environment that cause changes in DNA structure.