Write 4 sequential steps of the procedure of the experiment preparing a temporary mount of a leaf peel to show stomata
Answers
Answer:
1. Remove a healthy leaf from the potted plant.
2. Remove a part of the peel from the lower surface of the leaf. You can do this by folding the leaf over and gently pulling the peel apart using forceps. Keeps the peel in a watch glass containing water. Put a few drops of safranin stain in a watch glass.
3. After 2-3 minutes take out the peel and place it on a clean glass slide.
4. Put a drop of glycerin over the peel and place a clean coverslip gently over it with the help of a needle. Remove the excess stain and glycerin with the help of blotting paper. Observe the slide under the low-power and high-power magnifications of the compound microscope.
Explanation:
Four steps of preparing a temporary mount of leaf peel:
(1) Peel off the epidermis from the lower side of leaf by tearing it.
(2) place the peel in a glass containing water and then transfer the peel to water glass and add rafranin.
(3) Again transfer the peel back to glass to wash out excess stain. Lift the peel using needle for placing the peel on side.
(4) Pour a drop of glycerin on slide and place the peel on it and place a clean coverslip gently over it.
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