Write a note on cDNA library.
Answers
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Answer:
cDNA library or complementary DNA library is formed from mRNA by the help of Revers transcriptase enzyme.
Explanation:
There are 2 types of library in the molecular biology 1 cDNA library 2 Genomic library .
cDNA library cDNA library formation is an important application of genetic engineering.cDNA is formed from processed mRNA (intron free) by the help of reverstranscriptase enzyme .The mRNA is then removed from the medium by the treatment of alkaline phosphatase.Now the single stranded DNA is allowed to undergo reolication to form a double stranded DNA.The so formed double stranded DNA is treated with cognet EcoR1 to gererate sticky ends and these sticky DNA strands are used to clone in a suitable vector resuting the formation of recombinant DNA molecule.This recombinant DNA is then incorporated inside a suitable host.