Write – about Isolation of
new Antibiotics.
Answers
Answer:
Objective
To isolate and screen antibiotic producing actinomycetes from potential soil samples of Gondar town, Ethiopia.
Methods
Fifteen soil samples were collected, serially diluted and spread on starch casein and oat meal agar supplemented with amoxicillin and cyclohexamide for inhibition of bacteria and fungi, respectively. Cross streak method was used to check antagonistic activity of isolated actinomycetes against test organisms. Solid state fermentation and crude extraction were used for the production of antibiotics from isolates. Agar well diffusion was used for antimicrobial activity of crude extracts against test organisms.
Results
Three isolates (Ab18, Ab28 and Ab43) have been shown high antagonistic activity during primary screening. Inhibition zones obtained from crude extracts showed significance differences when compared with standard antibiotics tested against test organisms (P<0.05). Inhibition zone of crude extracts from isolate Ab18 against Klebsiella pneumonia ATCC7000603 and Escherichia coli ATCC25922 were (14±1) mm and (35±1) mm, respectively which were strong active when compared to amoxicillin (0 mm) and tetracycline [(13±1) mm for Klebsiella pneumonia ATCC7000603 and (33±1) mm for Escherichia coli ATCC25922]. Crude extracts from isolate Ab18 showed (20±1) mm and (15±1) mm inhibition zones against methicillin resistant Staphylococcus aureus strains 2 (MRSA2) and MRSA4, respectively
Answer:
first antibiotic penicillin
Explanation:
it it was discovered by Sir Alexander Fleming
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