Question

Write an account on fusion methods of hybridoma.

Answer 1

Myeloma cells cannot survive on HAT medium; spleen cells can, but are not immortal → only fused cells survive

Answer 2

Explanation:

The fusion of the B cells with myeloma cells can be done using electrofusion. Electrofusion causes the B cells and myeloma cells to align and fuse with the application of an electric field. Alternatively, the B-cells and myelomas can be made to fuse by chemical protocols, most often using polyethylene glycol.