Write down all the enzymes participating in DNA replication with their function write each one
Answers
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Enzymes Participating in DNA Replication :
❇ DNA - Dependent DNA Polymerase :
➡ It is Highly Efficient Enzyme With The abilities of 2000 bp per second .
➡ They Catalyse The Reaction With High Degree of Accuracy .
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❇ Deoxyribonucleoside Tri phosphate :
➡ This Enzyme Has Dual purpose .
➡ It act as Substrate and also provide Energy For polymerisation Reaction .
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❇ Helicase :
➡ Unwinding Of Helix .It is ATP dependent .
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❇ Topoisomerases :
➡ The Tension is Released By this Enzyme .
❇ DNA Ligase :
➡ The Discontinuous Fragment's are joined by the DNA ligase .
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Answer:
DNA polymerases
These enzymes copy DNA sequences by using one strand as a template. The reaction catalyzed by DNA polymerases is the addition of deoxyribonucleotides to a DNA chain by using dNTPs as substrates,
The best‐studied bacterium, E. coli, has three DNA polymerase types.
DNA polymerase I (Pol I) is primarily a repair enzyme, although it also has a function in replication
DNA polymerase II is a specialized repair enzyme. Like Pol I, a large number of Pol II molecules reside in the cell (about 100). The enzyme is more processive than Pol I. Pol II has the same editing (3′ to 5′) activity as Pol I, but not the 5′ to 3′ exonuclease activity.
The actual replication enzyme in E. coli is DNA polymerase III. Its properties contrast with Pol I and Pol II in several respects. Pol III is much more processive than the other enzymes, making about 500,000 phosphodiester bonds on the average.
PRIMASE -Chain initiation occurs when a specialized RNA polymerase enzyme called primase makes a short RNA primer.
DNA gyrase (topoisomerase II) helps unwind the DNA double helix and keep the double strands from tangling during replication
DNA ligase, seals off the nicks by using high energy phosphodiester bonds in ATP or NAD to join a free 3′ hydroxyl with an adjacent 5′ phosphate