Question

Write down the methods of introducton of rDNA inside the host cell?

Answer 1

The recombinant vector carrying foreign DNA needs to be transferred into the suitable host cell. These are several methods to introduce recombinant vectors and these are dependent on several factors such as the vector type and host cell.In rDNA technology the most common method to introduce rDNA into living cell are called transformation.However, in nature the frequency of transformation of many cells (example yeast and mammalian cells) is very less. Secondly, all the time host cells do not undergo transformation, because they are not prepared for it.When they develop competence factors in the cells, transformation phenomenon occurs.


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