Write down the steps of Plant tissue culture?
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Answer:
Plant tissue culture processes were initially developed and practiced in university and government-based laboratories. In recent years, however, the process has moved beyond these research facilities to widespread use among commercial enterprises as a cost-effective tool for plant propagation, new variety introductions and research.
Plant tissue culture has revolutionized the flower and nursery markets by making valuable new hybrid clones available in commercial quantities comparatively soon after their first discovery. It is possible to start with a single plantlet and, in the space of 10-12 months, create in excess of 250,000 identical copies or clones.
1.STAGE 1: Initiation phase. The initiation phase is the first phase of tissue culture. ...
2.STAGE 2: Multiplication stage. ...
3.STAGE 3: Root formation. ...
4.General procedure for plant tissue culture:
5.Medium preparation: ...
6.Plant preparation: ...
7.Transferring the plant material to a tissue culture medium: ...
8.Technique for Plant in Vitro Culture: