write the principal behind chromatography
Answers
Explanation:
Chromatography is an important biophysical technique that enables the separation, identification, and purification of the components of a mixture for qualitative and quantitative analysis. Therefore, there are three working principles.
1)Proteins can be purified based on characteristics such as size and shape, total charge, hydrophobic groups present on the surface, and binding capacity with the stationary phase.
2)Four separation techniques based on molecular characteristics and interaction type use mechanisms of ion exchange, surface adsorption, partition, and size exclusion.
3)Other chromatography techniques are based on the stationary bed, including column, thin layer, and paper chromatography. Column chromatography is one of the most common methods of protein purification.
Answer:
Chromatography is based on the principle of molecules in mixture applied onto the surface or into the solid, and fluid stationary phase( stable phase) is separating from each other while moving with the aid of mobile phase.