Question

WRITE THE PRINCIPLE AND APPLICATIONS OF THE FOLLOWING PROCESSES :
A : Chromatography
B : Centrifugation

Answer 1

A : Chromatography

principle : It is based on the principal of separating the components of the mixture by the differential movement of individual component through a stationary phase.This separation is based on the fact that through two or more substance are soluble in the same solvent but their solubility may be different.

Application : to separate

1. colors in a dye

2.pigment from natural colors

Centrifugation :

principle :The principle is that the denser particles are forced to the bottom and the lighter particles stay at the top.

Application:

1.Used in diagnostic lab for blood and urine test

2.used in dairies and home to separate butter from cream