write the procedure for section cutting of steam and slide preparation?
Answers
Answer:
To prepare the slide:
Place a drop of fluid in the center of the slide.
Position sample on liquid, using tweezers.
At an angle, place one side of the cover slip against the slide making contact with outer edge of the liquid drop.
Lower the cover slowly, avoiding air bubbles.
Remove excess water with the paper towel
Answer:
Take 2-3cm long pieces of the material.
Hold the material between thumb and first finger of your left hand.
Hold the razor in the right hand with edge of the blade facing you and handle at right angle to it.
Dip the top of the material in water.
Then start cutting transverse sections as fast as possible in a watch glass containing water.
Select the thinnest section of the material with the help of a delicate brush.
Take a clean watch glass with water, transfer thin sections of the material.
Put a few drops of saffranin stain in the watch glass with water.
Leave it for 3-5 minutes.
Drain off stain and wash with water if necessary.
Put the thinnest section in the centre of the slide.
Put a drop of glycerine over the material.
Cover it with a coverslip with the help of needle.
Observe it under a compound microscope after staining and mounting.