Question

Write the steps involve in preparing temporary mount of leaf peel to show stomata.

Answer 1

Answer:Objective:

To prepare a temporary mount of a leaf peel to show stomata.

Apparatus and Materials Required:

A potted Tradescantia or Bryophyllum plant, forceps, needles, watch glasses, glass slides, a dropper, coverslips, a brush, blotting paper, safranin, glycerine and a compound microscope.

Theory:Stomata are small openings found widely scattered on the epidermis of leaves and young stems. They are mostly found on the lower surface of a dicot leaf and on both the surfaces of a monocot leaf. Stomata regulate the exchange of gases and water vapour between the atmosphere and leaves.

Procedure:

1. Remove a healthy leaf from the potted plant.

2. Remove a part of the peel from the lower surface of the leaf. You can do this by folding the leaf over and gently pulling the peel apart using forceps. Keeps the peel in a watch glass containing water.

3. Put a few drops of safranin stain in a watch glass                                        4. After 2-3 minutes take out the peel and place it on a clean glass slide.

5. Put a drop of glycerin over the peel and place a clean coverslip gently over it with the help of a needle.

6. Remove the excess stain and glycerin with the help of blotting paper.

7. Observe the slide under the low-power and high-power magnifications of the compound microscope.

Explanation:

Answer 2

$\huge \red {\underline {HAYE\:MATE}}$

$\huge \red {\mathbb {ANSWER}}$

The steps are:

i. Removal of peel from leaf.

ii. Stain with safranin.

iii. Put the stained peel on a clean slide.

iv. Mount it with glycerine and cover slip.

$\huge \blue {\mathbb {THANKS}}$

$\huge \green {FOllOW\:ME}$

$\huge \purple {\mathbb {SINGLE\:STAR}}$