Write the steps that lead to aminoacylation of tRNA in the cell.
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The first step, termed 'activation,' is the formation of an aminoacyl-AMP (aminoacyl-adenylate) on the enzyme through the hydrolysis of ATP. The second step is the transfer of the activated amino acid residue from the adenylate to a tRNA in a reaction referred to as 'charging.
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Explanation:
The mechanism can be summarized in the following reaction series: Amino Acid + ATP → Aminoacyl-AMP + PP. Aminoacyl-AMP + tRNA → Aminoacyl-tRNA + AMP.
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