Write the steps you would suggest to undertaken to 10 foreign gene product
Answers
Answered by
0
okk foreign Gene product are Below
the steps to be undertaken to obtain a foreign-gene-product:
Step 1. On insertion of a piece of DNA into cloning vector and tranfer into a bacterial plant or animal cell, the alien DNA gets multiplied.
Step 2. After having cloned the gene of interest and having optimised the conditions to induce the expression of the target protein, one has to consider producing it on a large scale.
Step 3. The Cultures may be used for extracting the desired protein and then purifying it by using different separation technique.
Step 4. The cells can also be multiplied in a continous culture system. The used medium is drained out from one side, while fresh medium is added from the other to maintain the cells in their physiologically most active log exponential phase.
this what I know please try to find in books or Google, because this information is from my old book.... please follow me on my brainly account please
the steps to be undertaken to obtain a foreign-gene-product:
Step 1. On insertion of a piece of DNA into cloning vector and tranfer into a bacterial plant or animal cell, the alien DNA gets multiplied.
Step 2. After having cloned the gene of interest and having optimised the conditions to induce the expression of the target protein, one has to consider producing it on a large scale.
Step 3. The Cultures may be used for extracting the desired protein and then purifying it by using different separation technique.
Step 4. The cells can also be multiplied in a continous culture system. The used medium is drained out from one side, while fresh medium is added from the other to maintain the cells in their physiologically most active log exponential phase.
this what I know please try to find in books or Google, because this information is from my old book.... please follow me on my brainly account please
Similar questions