Question

You are using ammonium sulphate to purify protein Q (pI = 5.0) by salting out from a solution at

pH 7.0. How would you adjust the pH of the mixture to maximize the amount of protein Q that

precipitates?​

Answer 1

Answer: Lower the pH from 7.0 to 5

Explanation:

Because it would promote the precipitation of Q because the protein will be less soluble when its net charge is equal to 0 and pH = pI