Chemistry, asked by titin1234, 1 year ago

200 ml of aqueous solution of protein contain 1.26 of protein the osmotic pressure of such solution at 300 k is found to be 2.57×10^3. Calculate the molecular mass of protein....... please help urgent.....no spam......give satisfying answer...... thank u

Answers

Answered by sharpyy
57
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Answered by kobenhavn
13

Answer:  The molecular mass of protein is 60377.04g/mol

Explanation:

\pi =CRT if osmotic pressures are equal at the same temperature, concentrations must also be equal.

\pi = osmotic pressure  =2.57\times 10^{-3}atm

C= concentration

R= solution constant  = 0.0821 Latm/Kmol

T= temperature  = 300 K

For protein: 1.26 g of urea is dissolved in 200 ml of solution.

Molarity=\frac{\text{Mass of solute}\times 1000}{\text{Molar mass of solute}\times \text{volume of solution in ml}}

C_{protein}=\frac{1.26\times 1000}{200\times M_{protein}}

2.57\times 10^{-3}=\frac{1.26\times 1000}{M_{protein}\times 200}\times 0.0821\times 300K

M_{protein}=60377.04g/mol

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