Question

7Q: Which of the following statements are true regarding PCR?
(a) Primer extension occurs at 72°C
(b) Denaturation involves heating at 90 to 98°C
(c) Annealing involves the binding of primer between 40 to 600°C
(d) All of the above
A. Only A correct
B. Only B correct
C. Only C correct
D. Only D correct​

Answer 1

Answer:

i think B is that answer

Explanation:

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Answer 2

Answer:

Both the statements (a) and (b) are correct.

Explanation:

• A method for making several copies of a given DNA region in vitro is called polymerase chain reaction, or PCR (in a test tube rather than an organism).
• Taq polymerase, primers, template DNA, and nucleotides are the main components of a PCR reaction (DNA building blocks). DNA can be created by assembling the components in a tube with the cofactors the enzyme requires and putting them through repeated cycles of heating and cooling.
• The basic steps are:

1. Denaturation- Strong heating of the process causes the DNA strands to split or get denatured. For the following step, this gives a single-stranded template.
2. Annealing- To enable the primers to bind to their complementary sequences on the single-stranded template DNA, the reaction must be cooled.
3. Extension- Increase the reaction temperatures to allow Taq polymerase to stretch the primers and create additional DNA strands.

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