Question

A flat tissue engineered skin (thickness 2.9 mm) is attached to the bottom of a dish and cultured with cell media atop it (which has an oxygen concentration of 0.15 mol/m3). The diffusivity of oxygen in the skin is 2.20E-9 m2/s, and according to experiments that you’ve performed, you want to make sure that the concentration of oxygen in the device doesn’t fall below 0.065 mol/m3. (Below that concentration harms the cells.)

The rate of oxygen consumption is -2.5E-15 mol/[cell◦s]. What is the maximum cellularity (in cells/mL) that this tissue engineered skin can support?

Answer 1

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