Biology, asked by meena1252, 1 year ago

A highly selective and sensitive fluorescent probe for hypochlorous acid and its lysosome-targetable biological applications

Answers

Answered by cprincy2018
0

Highlights

A novel lysosome-targetable fluorescence probe has been rationally designed and synthesized.

The probe exhibits high selectivity and sensitivity for the detection of HOCl.

The probe functions as an excellent fluorescence probe for real-time imaging of HOCl in lysosomes.

Abstract

A novel lysosome-targetable fluorescence probe PT-1 based on a photoinduced electron transfer (PET) mechanism has been designed and synthesized. For comparison, the probe PT-2 without morpholine moiety as a lysosome-directing group was also synthesized and investigated. Probes PT-1 and PT-2 exhibited high selectivity, high sensitivity (with the detection limits down to the 10−10 M range) and response in real time (within 10 s) toward HOCl over other reactive oxygen species (ROS). Both PT-1 and PT-2 were cell permeable and enabled them to be used for monitoring of HOCl in living cells. Meanwhile, the probe PT-1 demonstrated an accurately lysosome-targeting ability, and was successfully applied to image of exogenous, endogenous produced HOCl in living cells. The success of subcellular imaging suggested that the probe PT-1 could be used in further applications for the investigation of biological functions and pathological roles of HOCl at organelle levels.

Graphical abstract

A novel lysosome-targetable fluorescence probe has been rationally designed and synthesized for sensitive and selective detection of HOCl. The probe functions as an excellent fluorescence probe for real-time imaging of HOCl in lysosomes.

Keywords

Fluorescent probe1,8-NaphthalimideHypochlorous acidPETLysosomeImaging

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