A highly selective and sensitive fluorescent probe for hypochlorous acid and its lysosome-targetable biological applications
Answers
Highlights
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A novel lysosome-targetable fluorescence probe has been rationally designed and synthesized.
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The probe exhibits high selectivity and sensitivity for the detection of HOCl.
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The probe functions as an excellent fluorescence probe for real-time imaging of HOCl in lysosomes.
Abstract
A novel lysosome-targetable fluorescence probe PT-1 based on a photoinduced electron transfer (PET) mechanism has been designed and synthesized. For comparison, the probe PT-2 without morpholine moiety as a lysosome-directing group was also synthesized and investigated. Probes PT-1 and PT-2 exhibited high selectivity, high sensitivity (with the detection limits down to the 10−10 M range) and response in real time (within 10 s) toward HOCl over other reactive oxygen species (ROS). Both PT-1 and PT-2 were cell permeable and enabled them to be used for monitoring of HOCl in living cells. Meanwhile, the probe PT-1 demonstrated an accurately lysosome-targeting ability, and was successfully applied to image of exogenous, endogenous produced HOCl in living cells. The success of subcellular imaging suggested that the probe PT-1 could be used in further applications for the investigation of biological functions and pathological roles of HOCl at organelle levels.
Graphical abstract
A novel lysosome-targetable fluorescence probe has been rationally designed and synthesized for sensitive and selective detection of HOCl. The probe functions as an excellent fluorescence probe for real-time imaging of HOCl in lysosomes.
Keywords
Fluorescent probe1,8-NaphthalimideHypochlorous acidPETLysosomeImaging