A linear double stranded DNA of 6 kb contained one Pvu II site and one Eco RI site and they are situated 3kb apart. This DNA was completely digested with Pvu II and Eco RI. The products were purified and added to an appropriately buffered reaction mixture containing dNTP mix, α32-P dATP, and Klenow fragment of E.coli DNA polymerase I. The klenow reaction products were analyzed by gel electrophoresis and autoradiography. How many bands you will observe in the autoradiogram?
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