According to Chargaff's rule, base pairing in DNA should resemble which of the following?
A = T; C = G
A = C; T = G
A = G; T = U
A = U; C = G
Answers
Chargaff's Rule of Base Pairing
The rules of base pairing (or nucleotide pairing) are:
A with T: the purine adenine (A) always pairs with
the pyrimidine thymine (T)
C with G: the pyrimidine cytosine (C) always pairs with
the purine guanine (G)
✓This is consistent with there not being enough space (20 Å) for two purines to fit within the helix and too much space for two pyrimidines to get close enough to each other to form hydrogen bonds between them.
The answer: only with A & T and with C & G are there opportunities to establish hydrogen bonds (shown here as dotted lines) between them (two between A & T;
three between C & G). The ability to form hydrogen bonds makes the base pairs more stable structurally.
These base pair relationships are often called Chargaff's rules of DNA base pairing, named after the Columbia University scientist.
The rules of base pairing tell us that if we can "read" the sequence of nucleotides on one strand of DNA, we can immediately deduce the complementary sequence on the other strand.
The C+G : A+T ratio varies from organism to organism among the prokaryotes), but within (particularly the limits of experimental error, A = T and C = G.
Relative Proportions (%)
Proportions (%) of Bases in DNA
Proportions (%) of Bases in DNAOrganism A T G C
Proportions (%) of Bases in DNAOrganism A T G CHuman 30.9 29.4 19.9 19.8
Proportions (%) of Bases in DNAOrganism A T G CHuman 30.9 29.4 19.9 19.8Chicken 28.8 29.2 20.5 21.5
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Answer:
A=G C=T
hope it helps....