Question

Advantages and disadvantages of ouchterlony double diffusion

Answer 1

Advantages of Ouchterlony Test1. See more than 1 Ag2. Qualitative test3. Can identify Agsa. line of identityb. nonidentityc. partial identityProblems1. TimeReaction occurs within this time frame 24-48 hours2. Sensitivity Depends on if Ab/Ag is in:Antibody-excess zone, Equivalence zone or Antigen-excess zoneOuchterlony Immunodiffusion.The method of double diffusion in a gel matrix, usually agarose, and the precipitation of antigens and antibodies that are loaded into these wells. Single Radial Immunodiffusion (Mancini Test).Is where either Ag or Ab is incorporated into gel v.s.•Affinity: Strength of interaction between epitope and one antigen-binding site•Avidity: Strength of the sum of interactions between antibody and antigenIt is important not to place the wells too far apart, otherwise you will not get the desired precipitation.


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