At 0°C, the enzymatic activity is
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At 0°C and 100°C, the rate of enzyme-catalyzed reactions is nearly zero.
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At 0°C enzyme action is low because the movement of molecules is low. This causes the collision frequency between enzyme and substrate to be low. ... As the temperature raises the shape of the enzyme changes and the enzyme becomes denatured. Temperature above 50 degrees Celsius will denature most human enzymes.
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