Question

Cells and cell organelles are too smell to be seen with naked eyes, so microscopes are used to observe these tiny units of life. In order to view the cells under microscope , cells are stained. Cell staining is a technique which is used to enhance the visibility of cell and certain components under a microscope. Preferential staining of cell or cellular components like nucleus and cell wall is carried out by using different stains. Most stains can be used on non-living cells while some can be used on living cells. Some stains can be used on both. There are several stains which are used for different purposes. Bismarck brown colours a particular protein in yellow colour and is used to stain the live cells. Carmine colours the glycogen in red colour. Malachite green is used to stain spores. Methylene blue stains animal cell to enhance the visibility of nucleus. Eosin is used to stain RBC, cell membrane and extracellular structure in pink colour while Fuschin stains mitochondria. Iodine is a starch indicator while crystal violet indicates the presence of cell wall by staining it purple. Staining depends on the type of analysis to be done. Cell may be stained to highlight the metabolic process or to differentiate between living and dead cells in a sample.
Rohan observes a tiny, blue-coloured spherical structure in the centre of the cell while

viewing a slide stained with methylene blue under compound microscope. Rohan is

observing a
(a) plant cell (b) animal cell

(c) bacterial cell (d) viral cell

(ii). Rohan puts iodine in the test tube containing the sample. The sample turns blue-black

on addition of iodine. What could be the sample in the test tube?

(a) sunflower oil (b) egg

(c) potato (d) any of these

(iii). Madan is using Bismarck brown to stain the specimen. It means that Madan is working

on a

(a) living cell (b) dead cell (c) either living or dead (d) virus

(iv). Neha is preparing a temporary mount of blood to observe the erythrocytes in it. Which

stain should she choose to have a clear picture of erythrocytes under the microscope?

(a) iodine (b) eosin (c) carmine (d) fuschin​

Answer 1

Answer:

1. Animal cell
2. patato
3. living cell
4. eosin