Describe in detail techniques of recombinant dna technology
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generation of DNA
insertion of DNA into vector
insertion of vector DNA into the host cell
multiplication
expression
insertion of DNA into vector
insertion of vector DNA into the host cell
multiplication
expression
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Hey dearie,
The steps of rDNA Technology are
1)Fishing out of desirable gene
desirable gene is removed out from total DNA by using a special enzyme -Restriction endonuclease
2) Identification of suitable vector
a vector is a molecular vehicle which transfer desirable gene into host cell
3)Construction of rDNA
recombinant DNA is the combination of desirable gene and vector.
4)Introduction of rDNA into host cell
5) Screening
The steps of rDNA Technology are
1)Fishing out of desirable gene
desirable gene is removed out from total DNA by using a special enzyme -Restriction endonuclease
2) Identification of suitable vector
a vector is a molecular vehicle which transfer desirable gene into host cell
3)Construction of rDNA
recombinant DNA is the combination of desirable gene and vector.
4)Introduction of rDNA into host cell
5) Screening
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