Describe role of agrobacterium tumficiens in transforming plant cell
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Agrobacterium-Mediated Transformation
Agrobacterium tumefaciens is a plant pathogenic bacterium that contains a plasmid (the tumour-inducing or Ti plasmid), part of which (the T-DNA) integrates into the host plant chromosomes (Box 1.7); it has been termed “nature’s genetic engineer”. For plant transformation, the tumour-inducing genes are removed to make a “disarmed” plasmid. The GOI is placed between the left and right borders of the T-DNA which is then reintroduced into Agrobacterium. This Agrobacterium is then co-cultivated with suitable target cells (embryogenic suspensions or callus) to enable the modified T-DNA to integrate into the cells. After removal of the Agrobacterium, the transformed cells are grown into plants under selection.
Agrobacterium tumefaciens is a plant pathogenic bacterium that contains a plasmid (the tumour-inducing or Ti plasmid), part of which (the T-DNA) integrates into the host plant chromosomes (Box 1.7); it has been termed “nature’s genetic engineer”. For plant transformation, the tumour-inducing genes are removed to make a “disarmed” plasmid. The GOI is placed between the left and right borders of the T-DNA which is then reintroduced into Agrobacterium. This Agrobacterium is then co-cultivated with suitable target cells (embryogenic suspensions or callus) to enable the modified T-DNA to integrate into the cells. After removal of the Agrobacterium, the transformed cells are grown into plants under selection.
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Plant Transformation Using Agrobacterium tumefaciens. Agrobacterium tumefaciens is a widespread naturally occurring soil bacterium that causes crown gall, and has the ability to introduce new genetic material into the plant cell (Gelvin, 2003). ... A Ti plasmid is a circular piece of DNA found in almost all bacteria.(❤hope this helps u..)
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