describe the carbobenzoxy method of synthesis of peptides
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Explanation:
The C-terminal residue is determined by the use of either a chemical reagent or the enzyme carboxypeptidase. The chemical reagent hydrazine forms aminoacyl hydrazides with every residue except the C terminus (Figure 3-10). The C terminus is thus readily identified by chromatographic procedures. The disadvantage of hydrazinolysis is that the entire sample is used to determine just one residue.
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