Question

Describe the principle of isoelectric focusing.

Answer 1

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❤Principle of Isoelectric Focusing (IEF) Isoelectric Focusing or IEF is a method of separating proteins according to their Isoelectric points in a pH gradient.

❤ Isoelectric point denoted as pI is defined as the pH at which protein carry no net charge, or pH at which protein become immobile in an electric field.

Answer 2

Principle of Isoelectric Focusing (IEF) Isoelectric Focusing or IEF is a method of separating proteins according to their Isoelectric points in a pH gradient. Isoelectric point denoted as pI is defined as the pH at which protein carry no net charge, or pH at which protein become immobile in an electric field.Isoelectric Focusing. Isoelectric focusing (IEF) is a high-resolution electrophoretic technique for separating proteins on the basis of their isoelectric points (pI) and, as a consequence, can be used to determine the range of isoelectric points of proteins in a mixture.