Question

Difference between agarose gel electrophoresis and polyacrilamide gel electrophoresis

Answer 1

The difference between Polyacrylamide Gel Electrophoresis(PAGE) and Agarose Gel Electrophoresis (AGE): PAGE is used mainly for the separation of proteins. AGE is generally used for the separation of DNA. In a polyacrylamide gel, the pore size of the gel is much smaller than agarose gel.

Answer 2

Difference between agarose gel elctrophoresis and polyacrylamide gel electrophoresis are:

1. POLYACRYLAMIDE GEL ELECTROPHORESIS is a cross-linked polymer of ACRYLAMIDE while, AGAROSE GEL ELECTROPHORESIS is made up of long chain of interlinked sugar.
2. POLYACRYLAMIDE GEL ELECTROPHORESIS is a potent neuro-toxic while,AGAROSE GEL ELECTROPHORESIS is non-toxic.
3. Staining in POLYACRYLAMIDE GEL ELECTROPHORESIS can be done after pouring of gel while, staining in AGAROSE GEL ELECTROPHORESIS  can be done before pouring of gel.