Science, asked by AyushLokhande4520, 1 year ago

Effect of matrix stiffness in mode-i and mode-ii test for gfrp and cfrp laminates

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Answered by pinky162
2
yit is well known as matrix stiffness regulates numerous cellular function such as spreading and migration, proliferation and stem cell differentiation, influencing many major biological process.
effect of matrix stiffness on chondrocyte cytoskeleton organisation were access by labelling the actin cytoskeleton chondrocyte cultured on PDMS matrices were washed twice with PBS and fixed with 4% paraformaldehyde for 10 min permeablized with 0.1% triton.
the machenical property of individual chondrocyte cultured on different matrix stiffness were measured over the central region of the cell for the spherical morphology and over the nucleus for the spread morphology by AFM
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