Question

Explain the method of introduction of alien dna into bacterial cells. 2

Answer 1

First the DNA must be extracted.It can be transmitted into bacterial cell by means of vector.The vector generally used is plasmid (extrachromosomal dna of bacteria).The foreign DNA is integrated into plasmid by means of restriction enzymes (to cut) and DNA ligase (to paste).The recombinant plasmid is then transferred into bacterial cell by mixing the rplasmid with bacterial cells in a medium with cacl2.The rplasmid can also be trasfected by using bacteriophage.

Answer 2

Method of alien DNA into bacterial cells

Explanation:

• Recomninant DNA is a particle of DNA that has been altered, either through hereditary recombination lab methods.  

• Techniques for bringing outsider DNA into have cell:  Electroporation:-   Since DNA is a hydrophilic particle, it can't go through cell layers. So as to drive microorganisms to take up the plasmid, the bacterial cells should initially be made 'skillful' to take up DNA. This is finished by treating them with a particular convergence of a divalent cation.

• Microinjection:-  Recombinant DNA is legitimately infused into the core of a creature cell.  

• Biolistics or quality weapon:-  In another strategy, reasonable for plants, cells are shelled with high speed miniaturized scale particles of gold or tungsten covered with DNA in a technique known as biolistics or quality weapon. What's more, the last strategy utilizes 'incapacitated pathogen' vectors, which when permitted contaminating the phone, moving the recombinant DNA into the host.