Question

Explain the principle. and procedure of The ( thin layer chromatography)​

Answer 1

Answer:

Chromatography works on the principle that different compounds will have different solubilities and adsorption to the two phases between which they are to be partitioned. Thin Layer Chromatography (TLC) is a solid-liquid technique in which the two phases are a solid (stationary phase) and a liquid (moving phase).

Thin Layer Chromatography Procedure

Before starting with the Thin Layer Chromatography Experiment let us understand the different components required to conduct the procedure along with the phases involved.

Thin Layer Chromatography

Plates – ready-made plates are used which are chemically inert and stable. The stationary phase is applied on its surface in the form of a thin layer. The stationary phase on the plate has a fine particle size and also has a uniform thickness.

Thin Layer Chromatography Chamber – Chamber is used to develop plates. It is responsible to keep a steady environment inside which will help in developing spots. Also, it prevents the solvent evaporation and keeps the entire process dust-free.

Thin Layer Chromatography Mobile phase – Mobile phase is the one that moves and consists of a solvent mixture or a solvent. This phase should be particulate-free. The higher the quality of purity the development of spots is better.

Thin Layer Chromatography Filter Paper – It has to be placed inside the chamber. It is moistened in the mobile phase.